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Laboratory methods
Laboratory methods for VTEC detection and typing
The laboratory methods for the detection, identification, and typing of VTEC and other pathogenic E.coli are available in this section.
A report on the performance data of the ISO/TS 13136 procedure for the detection of STEC/VTEC in food, based on the results of the PTs organized by the EU-RL, is also available. Such a report can be used as reference for the purpose of accreditation of the method.
Documents
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Identification and characterization of Verocytotoxin-producing Escherichia coli (VTEC) by PCR amplification of the main virulence genes (EU-RL VTEC_Method_01_Rev 0)
[PDF - 403.89 kbytes]
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Identification and characterization of Verocytotoxin-producing Escherichia coli (VTEC) by Real Time PCR amplification of the main virulence genes and the genes associated with the serogroups (EU-RL VTEC_Method_02_Rev 0)
[PDF - 270.97 kbytes]
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Identification of VTEC serogroups mainly associated with human infections by conventional PCR amplification of O-associated genes (EU-RL VTEC_Method_03_Rev 1)
[PDF - 303.60 kbytes]
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Detection and identification of Verocytotoxin-producing Escherichia coli (VTEC) O104:H4 in food by Real Time PCR (EU-RL VTEC_Method_04_Rev 1)
[PDF - 308.27 kbytes]
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Detection of Enteroaggregative Escherichia coli in food by Real Time PCR amplification of aggR and aaiC genes (EU-RL VTEC_Method_05_Rev 1)
[PDF - 298.93 kbytes]
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Identification of the subtypes of Verocytotoxin encoding genes (vtx) of Escherichia coli by conventional PCR (EU-RL VTEC_Method_06_Rev 1)
[PDF - 205.30 kbytes]
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Detection of Enteroinvasive Escherichia coli in food by Real Time PCR amplification of the ipaH gene (EU-RL VTEC_Method_07_Rev 0)
[PDF - 265.77 kbytes]
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Detection of Enterotoxigenic Escherichia coli in food by Real Time PCR amplification of the lt, sth, and stp genes (EU-RL VTEC_Method_08_Rev 0)
[PDF - 273.31 kbytes]
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Performance Parameters of the CEN ISO/TS 13136:2012 (EU-RL VTEC_Method_performance_CEN ISO/TS_13136:2012_Rev 4)
[PDF - 1925.67 kbytes]
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Laboratory procedure for testing spent irrigation water for the presence of STEC (EU-RL VTEC_Method_09_Rev 1)
[PDF - 46.70 kbytes]
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Detection of Escherichia coli producing the Stx2f subtype by Real-Time PCR (EU-RL VTEC_Method_10_Rev 0)
[PDF - 255.49 kbytes]
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Identification of the STEC serogroups mainly associated with human infections by Real-Time PCR amplification of O-associated genes (EU-RL VTEC_Method_11_Rev 0)
[PDF - 292.70 kbytes]
