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Proficiency Testing

Proficiency Testing on the molecular identification of Anisakid nematodes at the species level: aim, instructions and procedure

The EURLP organizes proficiency testing for laboratories performing official controls for foodborne parasites on food and feed, according to the ISO/IEC 17043:2010. This ISO standard defines the proficiency testing as evaluation of participant performance against pre-established criteria by means of interlaboratory comparisons. The EURLP organize Proficiency Testing (PT) for the molecular identification of Anisakid nematodes at the species level.
Who can participate to the PT. All the National Reference Laboratory (NRL) for parasites from EU member states or associated countries.
Aim of the PT. To correctly identify anisakid L3 larvae and DNA at species level by a molecular method.
PT item used. Four different species of Trichinella muscle larvae preserved in 96% ethanol.
PT Panel composition. The panel of samples consists of 4 tubes: 2 tubes containing a single fragment of Anisakidae L3 larva each; and 2 tubes containing DNA extracted from a single Anisakidae L3 larva. .
Procedure to analyze the panel of samples.The EURLP recommends performing the analysis using one of the EURLP methods“Identification at species level of parasites of the family Anisakidae by PCR/RFLP” or“Identification of Anisakidae Larvae at the species level by multiplex PCR”. Any other suitable molecular method performed by the participant laboratory can be applied.
Criteria for the result evaluation. Results evaluation is only qualitative, the participant have to correctly identify the larvae in the samples at species level. Final evaluation is considered as “positive” if all species are successfully identified.
Report. The EURLP provides both an Individual PT report and a Final PT report presented during the annual NRL workshop and afterwards published on the EURLP web site. To guarantee confidentiality, the individual PT report is available only to the participant lab, and in the final PT report, only lab codes are displayed. The PT Reports are retained by EURLP for 10 years.

Detailed instructions on the PT procedure are reported in the .pdf document you can download from below.


Published 22-02-2013 in Proficiency Testing , last update 07-03-2017


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