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Proficiency Testing

Proficiency Testing for the detection of Anisakidae L3 larvae in fish fillets: aim, instructions and procedure

The EURLP organizes proficiency testing for laboratories performing official controls for foodborne parasites on food and feed, according to the ISO/IEC 17043:2010. This ISO standard defines the proficiency testing as evaluation of participant performance against pre-established criteria by means of interlaboratory comparisons. The EURLP organize Proficiency Testing (PT) for the identification of L3 larvae of the nematode belonging to Anisakidae in the fish fillets.
Who can participate to the PTs. All the National Reference Laboratory (NRL) for parasites from EU member states or associated countries.
Aim of the PTs. Detection of Anisakidae larvae in fish fillet.
Procedure to analyse the panel of samples. A panel of 3 samples is delivered to the participants. Each sample consist of a fish sandwich spiked or not with Anisakidae L3 larvae free of their capsule. Samples shall be analyzed within 3 days after the delivery to the lab by one of the following methods: i) candling; ii) compressorium; iii) UV examination after freezing; iv) digestion.
Criteria for the result evaluation. Evaluation of the results is qualitative. The results are considered “correct” if the laboratory identify properly samples spiked or not with Anisakidae larvae, or “incorrect” in case of false positive or false negative. The participating laboratory has also to indicate the number of larvae detected in each sample.The EURLP, due to the low number of samples and size of the larvae, cannot apply any statistical parameter for the result evaluation. The final evaluation is “positive” if the results of all samples were correct or “negative” if at least one result was incorrect.
Report. The EURLP provides an Individual PT Report including the following information: i) the number of spiked larvae per sample; ii) the number of larvae detected by the laboratory in each sample; iii) the final evaluation and iv) recommendation based on the laboratory performance, and a Final PT report presented during the annual NRL workshop and afterwards published on the EURLP web site. To guarantee confidentiality, the individual PT report is available only to the participant lab, and in the final PT report, only lab codes are displayed. The PT Reports are retained by EURLP for 10 years.

Detailed instructions and procedure to participate to the Proficiency Testing for the detection of Anisakidae L3 larvae in fish fillet, are reported in the .pdf documents you can download from below.


Published 22-02-2013 in Proficiency Testing , last update 31-05-2017


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