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Proficiency Testing

Proficiency Testing for the detection of Echinococcus sp. adult worms in the intestinal mucosa of the definitive host: aim, instructions and procedure

The EURLP organizes proficiency testing for laboratories performing official controls for foodborne parasites on food and feed, according to the ISO/IEC 17043:2010. This ISO standard defines the proficiency testing as evaluation of participant performance against pre-established criteria by means of interlaboratory comparisons. The EURLP organize Proficiency Testing (PT) for the identification of adult worms belonging to Echinococcus sp. In the intestinal mucosa of the definitive host.
Who can participate to the PTs. All the National Reference Laboratory (NRL) for parasites from EU member states or associated countries.
Aim of the PTs. Correctly identify the presence of adult worms of Echinococcus sp. in a matrix made by intestinal mucosa.
Procedure to analyse the panel of samples. A panel of 3 samples is delivered to the participants. Each 12 ml sample consists of 8 ml of homogenized intestinal mucosa plus 4 ml of ethanol 70%, spiked or not with adult worms of Echinococcus sp. Samples will be analysed by the sedimentation and counting technique (SCT) within 7 days after the delivery to the lab.
Criteria for the result evaluation. Results evaluation is performed only by qualitative approach. The results of the SCT are reported as “correct” or “incorrect”, irrespective of the number of worms in the sample/s. Final evaluation is considered as “positive” if all spiked samples are successfully detected.
Report. The EURLP provides both an Individual PT Report including the results and comments and/or recommendations made by EURLP and a Final PT Report presented during the annual NRL workshop and afterwards published on the EURLP web site. To guarantee confidentiality, the individual PT report is available only to the participant lab, and in the final PT report, only lab codes are displayed. The PT Reports are retained by EURLP for 10 years.

Detailed instructions on the PT procedure are reported in the .pdf document you can download from below.

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Published 22-02-2013 in Proficiency Testing , last update 07-02-2017

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