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Proficiency Testing

Proficiency Testing on the identification of Trichinella larvae at the species level by a molecular method: aim, instructions and procedure

The EURLP organizes proficiency testing for laboratories performing official controls for foodborne parasites on food and feed, according to the ISO/IEC 17043:2010. This ISO standard defines the proficiency testing as evaluation of participant performance against pre-established criteria by means of interlaboratory comparisons. The EURLP organize Proficiency Testing (PT) for the identification of Trichinella muscle larvae at species level.
Who can participate to the PT. All the National Reference Laboratory (NRL) for parasites from EU member states or associated countries.
Aim of the PT. Correctly identify Trichinella muscle larvae at species level by a molecular method.
PT item used. Four different species of Trichinella muscle larvae preserved in 96% ethanol.
PT Panel composition. Two alternative panels of samples can be requested by participants. The first panel consists of 4 tubes, each tube containing a pool of 10 larvae of the same species. The second panel consists of 12 tubes, 3 tubes for each species, each tube containing one single larva.
Procedure to analyze the panel of samples.Samples can be analyzed by any molecular method available in the lab, or by the EURLP method.
Criteria for the result evaluation. Results evaluation is only qualitative, the participant have to correctly identify the larvae in the samples at species level. Final evaluation is considered as “positive” if all species are successfully identified.
Report. The EURLP provides a PT report for the participant, also including the overall results. To guarantee confidentiality, the complete PT report (including the overtime evaluation) is available only to the participant, and in the overall summary of performance only the lab codes are displayed. The PT Reports are retained by EURLP for 10 years.

Detailed instructions on the PT procedure are reported in the .pdf document you can download from below.

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Published 22-02-2013 in Proficiency Testing , last update 07-02-2017

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