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Personale dell'ISS

Stefano Fais

Dirigente di ricerca
+39 06 4990 3195
Viale Regina Elena 299
00161 - Roma (I)
stefano.fais@iss.it


Curriculum vitae

Note Biografiche

laurea in medicina e chirurgia 30/10/1981, presso la Facoltà di medicina e Chirurgia Università degli Studi La Sapienza Roma
specializzazioni in Gastroenterologia e Patologia Generale, preso la stessa Università
Dottorato di Ricerca in Scienze Gastroenterologiche e Post-dottorao in Virologia, presso la stessa Università

Incarichi

Professore a contratto presso la Scuola di Specializzazione in Gastroenterologia, Facoltà di Medicina, Università di Catanzaro.

Attività di Ricerca

IMMUNITA' DELLA MUCOSA INTESTINALE UMANA E SUA RELAZIONE CON LA PATOGENESI DELLE MALATTIE INFIAMMATORIE CRONICHE DELL'INTESTINO(1980-1993)
PATOGENESI DELL'INFEZIONE DA HIV-1 (1992-1994)
USO DEI MODELLI DEI TOPI SCID UMANIZZATI NELLO STUDIO DELLA PATOGENESI DELL'HIV-1 (1994-1998)
USO DEI MODELLI DEI TOPI SCID UMANIZZATI NELLO STUDIO DEI TUMORI UMANI (1998-2003)
STUDIO DI NUOVI MECCANISMI DI EVASIONE TUMORALE (1998-2003)
STUDIO DELL'ASSOCIAZIONE MEMBRANA/CITOSCHELETRO NELL'APOPTOSI ED IN ALTRE FUNZIONI CELLULARI (1998-2003)
STUDIO DEI MECCANISMI DI FARMACORESISTENZA (1998-2003)

Progetti di ricerca

1.Localizzazione cellulare di fas e fasl e apoptosi nella cellula di melanoma (Progetto 1%, Ruolo dell’apoptosi in bioterapie innovative, Responsabile scientifico: Marco Pierotti, Istituto Nazionale per la Cura e lo Studio dei Tumori, Milano).
2. Ruolo del citoscheletro actinico nell’infezione da hiv-1, nella apoptosi virus-indotta fas-dipendente, nella risposta immune specifica e naturale all’infezione e nella resistenza ai farmaci antivirali, Progetto nazionale AIDS.
3.Establishing a pre-clinical in vivo model of immunotherapy in SCID mice(PROGETTO COORDINATO AIRC: Identification of new human tumor antigens and strategies to enhance their immunogenicity and override tumor escape. Responsabile scientifico: Dr. Giorgio Parmiani, Istituto Nazionale per la Cura e lo Studio dei Tumori, Milano).
4.Studi sull’attivita’ cannibalica dei tumori umani. Possibile ruolo nell’evasione tumorale ed approcci terapeutici innovativi.” 502 ISS.
5.Ruolo dell’attivita’ lisosomale dei melanosomi nella armacoresistenza dei melanomi umani (Progetto 1%, responsabile Dr G. Arancia, ISS)

Incarichi Istituzionali

membro della commissione delle linee guida per la terapia cellulare somatica
esperto EMEA 2005-2005-2006

Brevetti

1. METHOD FOR TUMOR EVALUATION, IDENTIFICATION OF ANTI-TUMOR TARGETS, AND TREATMENT”, by IMED AB, box 7710, S-103 95 Stockholm, SWEDEN; Inventors: Stefano Fais, Walter Malorni, Luana Lugini and Paola Matarrese (ISS, Rome, Italy). Provisional US patent application, deposit July 14, 2003.

2. “PHARMACEUTICAL COMPOSITIONS BASED ON MUTANTS CREATED BY INSERTIONS AND DELETIONS OF EZRIN”, by Istituto Superiore di Sanità Rome Italy; Inventors: Stefano Fais and Francesco Lozupone, Istituto Superiore di Sanità, Rome Italy. UK patent application n: 0318913.1, deposit 14 August 2003.

3. ”NOVEL USE FOR PROTON PUMP INHIBITORS”, by Istituto Superiore di Sanità Rome Italy; Inventors: Stefano Fais and FrancescA Luciani, Istituto Superiore di Sanità, Rome Italy. Filed with the British Patent Office on 12 February 2004.

Pubblicazioni

  1. Fais S, De Milito A, Lozupone F. The role of FAS to ezrin association in FAS-mediated apoptosis. Apoptosis: an international journal on programmed cell death 2005;10(5):941 - 947.
  2. Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L. Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape. Gastroenterology 2005;128(7):1796-1804.
  3. Luciani F, Matarrese P, Giammarioli AM, Lugini L, Lozupone F, Federici C, Iessi E, Malorni V, Fais S. CD95/phosphorylated ezrin association underlies HIV-1 GP120/IL-2 induced susceptibility to CD95(APO-1/Fas)-mediated apoptosis of human resting CD4+T-lymphocytes. Cell death and differentiation 2004;11(5):574-582.
  4. Lozupone F, Pende D, Burgio VL, Castelli C, Spada M, Venditti M, Luciani F, Lugini L, Federici C, Ramoni C, Rivoltini L, Parmiani G, Belardelli F, Rivera P, Marcenaro S, Moretta L, Fais S. Effect of human natural killer and gammadelta T cells on the growth of human autologous melanoma xenografts in SCID mice. Cancer research 2004;64(1):378-385.
  5. Migliaccio G, Testa U, Cometa MF, Fais S, Chistolini P, Bignami M, Agrimi U, Proietti E, Coccia EM. Linee guida sui prodotti per terapia cellulare. Notiziario dell'Istituto Superiore di Sanità 2004;17(07-08):9-14.
  6. Luciani F, Spada M, De Milito A, Molinari A, Rivoltini L, Montinaro A, Marra M, Lugini L, Logozzi M, Lozupone F, Federici C, Iessi E, Parmiani G, Arancia G, Belardelli F, Fais S. Effect of proton pump inhibitor pretreatment on resistance of solid tumors to cytotoxic drugs. Journal of the National cancer institute 2004;96(22):1702-1713.
  7. Lozupone F, Lugini L, Matarrese P, Luciani F, Federici C, Iessi E, Margutti P, Stassi G, Malorni W, Fais S. Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin. Journal of biological chemistry 2004;279(10):9199-9207.
  8. Lozupone F, Rivoltini L, Luciani F, Venditti M, Lugini L, Cova A, Squarcina P, Parmiani G, Belardelli F, Fais S. Adoptive transfer of an anti-mart1 27-35-specific CD8+ T cell clone leads to immunoselection of human melanoma antigen-loss variants in SCID mice. European journal of immunology 2003;33(2):556-566.
  9. Fais S, Malorni V. Leukocyte uropod formation and membrane/cytoskeleton linkage in immune interactions. Journal of leukocyte biology 2003;73:556-563.
  10. Lugini L, Lozupone F, Matarrese P, Funaro C, Luciani F, Malorni V, Rivoltini L, Castelli C, Tinari A, Piris A, Parmiani G, Fais S. Potent phagocytic activity discriminates metastatic and primary human malignant melanomas: a key role of Ezrin. Laboratory investigation 2003;83(11):1555-1567.
  11. Santini SM, Lapenta C, Logozzi M, Parlato S, Spada M, Di Pucchio T, Fais S, Belardelli F. Chimeric models of SCID mice transplanted with human cells: the Hu-PBL-SCID mouse and its use in AIDS research. In: Cossarizza A, Kaplan D, ed. Cellular aspects of HIV infection New York: John Wiley & Sons Inc; 2002. p.385-398.
  12. Ramoni C, Luciani F, Spadaro F, Lugini L, Lozupone F, Fais S. Differential expression and distribution of ezrin, radixin and moesin in human natural killer cells. European journal of immunology 2002;32(11):3059-3065.
  13. Travaglione S, Falzano L, Fabbri A, Stringaro A, Fais S, Fiorentini C. Epithelial cells and expression of the phagocytic marker CD68: scavenging of apoptotic bodies following Rho activation. Toxicology in vitro 2002;16(4):405-411.
  14. Rivoltini L, Carrabba M, Huber V, Castelli C, Novellino L, Dalerba P, Mortarini R, Arancia G, Anichini A, Fais S, Parmiani G. Immunity to cancer: attack and escape in T lymphocyte-tumor cell interaction. Immunological reviews 2002;188(1):97-113.
  15. Andreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S. Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles. Journal of experimental medicine 2002;195(10):1303-1316.
  16. Luciani F, Molinari A, Lozupone F, Calcabrini A, Lugini L, Stringaro A, Puddu P, Arancia G, Cianfriglia M, Fais S. P-glycoprotein-actin association through ERM family proteins: a role in P-glycoprotein function in human cells of lymphoid origin. Blood 2002;99(2):641-648.
  17. Fabbri A, Falzano L, Travaglione S, Stringaro A, Malorni V, Fais S, Fiorentini C. Rho-activating Escherichia coli cytotoxic necrotizing factor 1: macropinocytosis of apoptotic bodies in human epithelial cells. International journal of medical microbiology 2002;291(6-7):551-554.
  18. Fiorentini C, Falzano L, Fabbri A, Stringaro A, Logozzi M, Travaglione S, Contamin S, Arancia G, Malorni V, Fais S. Activation of Rho GTPases by cytotoxic necrotizing factor 1 induces macropinocytosis and scavenging activity in epithelial cells. Molecular biology of the cell 2001;12(7):2061-2073.
  19. Parlato S, Santini SM, Lapenta C, Di Pucchio T, Logozzi M, Spada M, Giammarioli AM, Malorni V, Fais S, Belardelli F. Expression of CCR-7, MIP-3beta, and Th-1 chemokines in type I IFN-induced monocyte-derived dendritic cells: importance for the rapid acquisition of potent migratory and functional activities. Blood 2001;98(10):3022-3029.
  20. Rizza P, Fais S, Pini C, Proietti E, Belardelli F. First international workshop on human/SCID mouse models. Journal of biological regulators and homeostatic agents 2001;15(2):170-174.
  21. Giammarioli AM, Garofalo T, Sorice M, Misasi R, Gambardella L, Gradini R, Fais S, Pavan A, Malorni V. GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein. FEBS letters 2001;506(1):45-50.
  22. Rizza P, Fais S, Pini C, Proietti E, Belardelli F. L'impiego dei modelli animali in campo biomedico: i modelli chimerici uomo-topo. Notiziario dell'Istituto Superiore di Sanità 2001;14(10):11-15.
  23. Arancia G, Molinari A, Calcabrini A, Stringaro A, Luciani F, Lozupone F, Luigini L, Puddu P, Cianfriglia M, Fais S. P-glycoprotein/actin association through erm family proteins: implication for development of multidrug resistance. In: Dini L, Catalano M, ed. 5. Multinational Congress on Electron Microscopy. Proceedings; September 20-25, 2001; Lecce. 2001. p.157-158.
  24. Proietti E, Fais S, Belardelli F, ed. 1. International workshop. Human/scid mouse models. Abstract book. Roma: Istituto Superiore di Sanità; 2000. (ISTISAN Congressi 00/70).
  25. Parlato S, Giammarioli AM, Logozzi M, Lozupone F, Matarrese P, Luciani F, Falchi M, Malorni V, Fais S. CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway. EMBO journal 2000;19(19):5123-5134.
  26. Fais S, Luciani F, Logozzi M, Parlato S, Lozupone F. Linkage between cell membrane proteins and actin-based cytoskeleton: the cytoskeletal-driven cellular functions. Histology and Histopathology 2000;15(2):539-549.
  27. Lozupone F, Luciani F, Venditti M, Rivoltini L, Pupa S, Parmiani G, Belardelli F, Fais S. Murine granulocytes control human tumor growth in scid mice. International journal of cancer 2000;87(4):569-573.
  28. Parlato S, Santini SM, Lapenta C, Spada M, Logozzi M, Rizza P, Proietti E, Belardelli F, Fais S. Primary HIV-1 infection of human CD4+ T cells passaged into SCID mice leads to selection of chronically infected cells through a massive Fas-mediated autocrine suicide of uninfected cells. Cell death and differentiation 2000;7(1):37-47.
  29. Fais S. Tecniche immunocitochimiche PAP-APAAP per la identificazione di pathways differenziativi cellulari. In: Molinari A, Arancia G, ed. Le tecniche microscopiche nello studio delle caratteristiche strutturali e funzionali delle cellule in coltura Roma: Centro Stampa De Vittoria; 2000. p.43-45.
  30. Fais S, Lapenta C, Santini SM, Spada M, Parlato S, Logozzi M, Rizza P, Belardelli F. Human immunodeficiency virus type 1 strains R5 and X4 induce different pathogenic effects in hu-PBL-SCID mice, depending on the state of activation/differentiation of human target cells at the time of primary infection. Journal of virology 1999;73(8):6453-6459.
  31. Lapenta C, Boirivant M, Marini L, Santini SM, Logozzi M, Viora M, Belardelli F, Fais S. Human intestinal lamina propria lymphocytes are naturally permissive to HIV-1 infection. European journal of immunology 1999;29:1202-1208 .
  32. Puddu P, Fais S, Luciani F, Gherardi G, Dupuis ML, Romagnoli G, Ramoni C, Cianfriglia M, Gessani S. Interferon-gamma up-regulates expression and activity of P-glycoprotein in human peripheral blood monocyte-derived macrophages. Laboratory investigation 1999;79(10):1299-1309.
  33. Rozera C, Mecchia M, Gresser I, Bandu MT, Proietti E, Venditti M, Sestili P, Santini SM, Fais S, Belardelli F, Ferrantini M. Murine interferon-alpha1 gene-transduced ESb tumor cells are rejected by host-mediated mechanisms despite resistance of the parental tumor to interferon-alpha/beta therapy. Cancer gene therapy 1999;6(3):246-253.
  34. Monini P, Colombini S, Stürzl M, Goletti D, Cafaro A, Sgadari C, Buttò S, Franco M, Leone P, Fais S, Leone P, Melucci-Vigo G, Chiozzini C, Carlini F, Ascherl G, Cornali E, Zietz C, Ramazzotti E, Ensoli F, Andreoni M, Pezzotti P, Rezza G, Yarchoan R, Gallo RC, Ensoli B. Reactivation and persistence of human herpesvirus-8 infection in B cells and monocytes by Th-1 cytokines increased in Kaposi's sarcoma. Blood 1999;93(12):4044-4058.
  35. Monini P, Stürzl M, Goletti D, Cafaro A, Sgadari C, Buttò S, Franco M, Leone P, Fais S, Leone P, Melucci-Vigo G, Chiozzini C, Carlini F, Andreoni M, Pezzotti P, Rezza G, Ensoli B. Reactivation and persistence of human herpesvirus-8 infection in B cells and monocytes by TH-1 cytokines increased in Kaposi's sarcoma. In: Centro di coordinamento, organizzazione e verifica dei progetti per la lotta all'AIDS, ed. Second national research program on AIDS. Progress report. Istituto Superiore di Sanità. Rome, July 12-16,1999. Roma: Istituto Superiore di Sanità; 1999. (Rapporti ISTISAN 99/11). p.326.
  36. Lapenta C, Santini SM, Parlato S, Logozzi M, Spada M, Rizza P, Fais S, Proietti E, Belardelli F. The "added value" of using hu-scid mouse models for studies on the pathogenesis, therapy and prophylaxis of HIV-1 infection". In: Centro di coordinamento, organizzazione e verifica dei progetti per la lotta all'AIDS, ed. Second national research program on AIDS. Progress report. Istituto Superiore di Sanità. Rome, July 12-16,1999. Roma: Istituto Superiore di Sanità; 1999. (Rapporti ISTISAN 99/11). p.349.
  37. Lapenta C, Santini SM, Proietti E, Rizza P, Logozzi M, Spada M, Parlato S, Fais S, Pitha PM, Belardelli F. Type 1 interferon is a powerful inhibitor of in vivo HIV-1 infection and preserves human CD4+ T cells from virus-induced depletion in SCID mice transplanted with human cells. Virology 1999;263:78-88.
  38. Rozera C, Mecchia M, Gresser I, Bandu MT, Proietti E, Venditti M, Sestili P, Santini SM, Fais S, Belardelli F, Ferrantini M. Murine interferon-alfa1 gene transduced ESb tumor cells are rejected by host-metiated mechanisms despite the resistance of the parental tumor to interferon-alfa/beta therapy. Cancer gene therapy 1999;6(3):246-253.
  39. Santini SM, Spada M, Parlato S, Logozzi M, Lapenta C, Proietti E, Belardelli F, Fais S. Treatment of severe combined immunodeficiency mice with anti-murine granulocyte monoclonal antibody improves human leukocyte xenotransplantation. Transplantation 1998;65(3):416-420.
  40. Lapenta C, Parlato S, Spada M, Santini SM, Rizza P, Logozzi M, Proietti E, Belardelli F, Fais S. Human lymphoblastoid CD4(+) T cells become permissive to macrophage-tropic strains of human immunodeficiency virus type 1 after passage into severe combined immunodeficient mice through in vivo upregulation of CCR5: in vivo dynamics of CD4(+) T-cell differentiation in pathogenesis of AIDS. Journal of virology 1998;72(12):10323-10327.
  41. Lapenta C, Parlato S, Spada M, Santini SM, Rizza P, Logozzi M, Proietti E, Belardelli F, Fais S. Human lymphoblastoid CD4(+) T cells become permissive to macrophage-tropic strains of human immunodeficiency virus type 1 after passage into severe combined immunodeficient mice through in vivo upregulation of CCR5: in vivo dynamics of CD4(+) T-cell differentiation in pathogenesis of AIDS. Journal of virology 1998;72(12):10323-10327.
  42. Lapenta C, Parlato S, Spada M, Santini SM, Rizza P, Logozzi M, Proietti E, Belardelli F, Fais S. Human lymphoblastoid CD4(+) T cells become permissive to macrophage-tropic strains of human immunodeficiency virus type 1 after passage into severe combined immunodeficient mice through in vivo upregulation of CCR5: in vivo dynamics of CD4(+) T-cell differentiation in pathogenesis of AIDS. Journal of virology 1998;72(12):10323-10327.
  43. Malorni V, Fais S, Fiorentini C. Morphological aspects of apoptosis. In: Boraschi D, Bossù P, Cossarizza A, ed. Apoptosis. A laboratory manual of experimental methods L'Aquila: Graphic Press; 1998.
  44. Monini P, Goletti D, Cafaro A, Sgadari C, Stürzl M, Buttò S, Franco M, Leone P, Fais S, Leone P, Ensoli F, Fiorelli V, Sirianni MC, Ensoli B. In vivo presence and in vitro transmission of human herpesvirus-8 (HHV-8) infection B cells, T cells, and monocytes-macrophages: viral activation by inflammatory cytokines (IC). Roma: Istituto Superiore di Sanità; 1997. (Rapporti ISTISAN 97/30). p.27.
  45. Fais S, Burgio VL, Capobianchi MR, Gessani S, Pallone F, Belardelli F. The biological relevance of polykaryons in the immune response. Immunology today (Reference ed.) 1997;18(11):522-527.
  46. Lapenta C, Fais S, Rizza P, Spada M, Logozzi M, Parlato S, Santini SM, Pirillo MF, Belardelli F, Proietti E. U937-SCID mouse xenografts: a new model for acute in vivo HIV-1 infection suitable to test antiviral strategies. Anticancer research 1997;36(2):81-90.
  47. Fais S, Borghi P, Gherardi G, Logozzi M, Belardelli F, Gessani S. Human immunodeficiency virus type 1 induces cellular polarization, intercellular adhesion molecule-1 redistribution, and multinucleated giant cell generation in human primary monocytes but not in monocyte-derived macrophages. Laboratory investigation 1996;75(6):783-790.
  48. Rizza P, Santini SM, Logozzi M, Lapenta C, Sestili P, Gherardi G, Lande R, Spada M, Parlato S, Belardelli F, Fais S. T-cell dysfunctions in hu-PBL-SCID mice infected with human immunodeficiency virus (HIV) shortly after reconstitution: in vivo effects of HIV on highly activated human immune cells. Journal of virology 1996;70(11):7958-7964.
  49. Fais S, Pallone F. Inability of normal human intestinal macrophages to form multinucleated giant cells in response to cytokines. Gut 1995;37(6):798-801.
  50. Santini SM, Rizza P, Logozzi M, Sestili P, Gherardi G, Lande R, Lapenta C, Belardelli F, Fais S. The scid mouse reaction to human peripheral blood mononuclear leukocyte engraftment. Transplantation 1995;60(11):1306-1314.
  51. Fais S, Maiuri L, Pallone F, De Vincenzi M, De Ritis G, Troncone R, Auricchio S. Gliadin induced changes in the expression of MHC-class II antigens by human small intestinal epithelium. Organ culture studies with coeliac disease mucosa. Gut 1992;33(4):472-475.

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